Requirements For Reading 1. Sample preparation 1) Sample: Human IgG tagged with HRP 2) Reagent: ECL+ 3) Conditions: One hour after the reaction 2. Detection conditions 1) Lens aperture: f=0.85 2) Exposure time: 10min 3) Sample tray position: 3 4) Dark frame subtraction: YES 5) Flat frame correction: No 6) Binning: No 3. Image contrast adjustment 1) Image type: Negative 2) Range: 0-2199