Requirements For Reading FLA2000 image 1. Sample preparation 1) Sample: Lambda DNA/HindIII 2) Reagent: SYPRO Orange 3) Conditions: One hour staining 2. Detection conditions 1) Pixel size: 50micron 2) Sensitivity: 100 3) Latitude: 5 4) Excitation wavelength: 473nm 5) Emission filter: Y520